Interferon-gamma is an important immunoregulatory cytokine that plays a key role in host defense by exerting antiviral, antiproliferative and immunoregulatory activities. Bovine IFNg is synthesized as 166 amino acid (aa) precursor that contains a 20aa signal sequence and a 146aa mature region. Based on human studies, bovine IFN-g exists as an a-helical, noncovalently linked intercalated homodimer. In the mature segment, bovine IFNg is 61%, 79%, 97%, 45%, 44%, and 55% aa identical to human, porcine, ovine, rat, mouse, and cotton rat IFNg, respectively. The production of IFNg is upregulated synergistically by IL-12, IL-18, IL-23, and IL-27. IFNg induces cytokine release and promotes the expression of various membrane proteins. This includes class I and II MHC antigens, Fc receptors, and leukocyte adhesion molecules. IFNg is a potent activator of macrophage effector functions. It also inhibits Th2 differentiation, stimulates Th1 development, and influences mononuclear cell chemotaxis. It influences chemotaxis by inducing the synthesis of CXC chemokines 9 and 10, and CC chemokines 2, 3, 4, and 5. The functional IFNg receptor complex consists of two distinct subunits. An alpha subunit (IFNg R1) binds IFNg with high affinity, while a b-subunit (IFNg R2) interacts with the IFNg occupied a-subunit to transduce a signal. Each IFN-g dimer interacts with two alpha- beta subunit receptor pairs.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Use 10ul to label 5x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.