Insulinlike Growth Factor I, is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumor cell growth.
IGFI signals through binding to the type 1 insulinlike growth factor receptor (IGF1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. IGFI production is stimulated by growth hormone (GH). and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
Recombinant corresponding to human IGF-I expressed in E.coli.
Molecular Weight: ~7.6kD
The ED50 was determined by a cell proliferation assay using FDCP1 cells is < 2.0 ng/ml, corresponding to a specific activity of >5 x 105 units/mg.
Suitable for use in Functional Assays. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O to 0.1-1mg/ml. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.