Inhibitor of Apoptosis Protein Detection Kit, Antibody Set, BioAssay(TM) (cIAP, ILP-2, Livin, NAIP, Survivin, XIAP)

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells (reviewed in 1). This process can be interrupted by a family of proteins termed inhibitors of apoptosis (IAP). Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR). These BIRs promote protein-protein interactions with members of the TRAF family of signaling molecules as well as caspases such as caspase-3, -7, and -9, and inhibiting the apoptotic activity of these proteins (reviewed in 2). Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy (3). Other IAPs include the X-linked protein XIAP/ILP-1 (4), the related cIAP protein which exists in two distinct isoforms (5), Livin (also known as KIAP as it is highly expressed in kidney) (6), Survivin/TIAP (7), ILP-2 (8), and Bruce, an extremely large IAP with ubiquitin-conjugating enzyme activity (9). Upregulation of these proteins inhibit the normal apoptotic process and thus may be involved in human diseases such as cancer. Kit Components: cIAP antibody (50ug). ILP-2 antibody (50ug). Livin antibody (50ug). NAIP antibody (50ug). Survivin antibody (CT) (50ug). XIAP antibody (50ug). Source: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins. Applications: These polyclonal antibodies can be used for detection of cIAP, ILP-2, Livin, NAIP, Survivin, and XIAP by immunoblot at 1-2ug/ml. These antibodies can also be used at 2-10ug/ml to detect their respective proteins via immunohistochemistry / immunocytochemistry. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.