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Immunohistochemistry HRP Detection Kit, BioAssay(TM)

Cat no: I7506-02

Supplier: United States Biological
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The Immunohistochemistry HRP Detection Kit features proprietary and enhanced HRP polymer technology to provide excellent sensitivity, high specificity, and a shorter protocol than conventional immunohistochemical staining methods. New polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. Polymer conjugate contains multiple HRPs to provide excellent sensitivity and specificity. The polymer staining method is faster and easier than a conventional LAB-SA protocol because it uses an HRP polymer conjugated to the second antibody and eliminates one incubation step. In addition, this kit does not contain biotin nor streptavidin, so potential background due to endogenous biotin activity is completely avoided. Another advantage is the shorter polymer incubation time; the kit only requires 10 mintues, while leading competitors polymers require 30 minutes. Detection kit is designed to reveal antigens that have reacted with a user-supplied primary antibody on human tissue or cell samples. Frozen or paraffin-embedded tissues, freshly prepared lymphocytes, and fixed cultured cells can be used. Species Crossreactivity: Recognizes mouse, rabbit, guinea pig and rat primary antibodies. Kit Components: I7506-02A. HRP polymer conjugate 1x15ml I7506-02B. AEC Solution 1x15ml Note: AEC is alcohol soluble. Do not dehydrate. Storage and Stability: Store at 4 degrees C. Stable for at least 6 months.
Catalogue number: I7506-02
Reactivities: Mouse, Rat, Guinea Pig, Rabbit
Applications: Immunohistochemistry
Size: 1Kit
References: 1. Petrosyan, K., et al., J. Histotechnology 25(4): 247-250 (2002).

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