IL-13 is a 17kD immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils. IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four A-helix configuration. Mature cotton rat IL-13 shares 59%, 74%, and 72% aa sequence identity with human, mouse, and rat IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat. IL-13 has diverse activities on numerous cell types. On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, up-regulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 up-regulates IL-6 while down-regulating IL-1 and TNF-A production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 RA1, triggering IL-13 RA1 association with IL-4 RA. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex. Additionally, IL-13 binds with high affinity to IL-13 RA2 which is expressed intracellularly, on the cell surface, and as a soluble molecule. IL-13 RA2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies. Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 RA2.
Recombinant corresponding to aa1-133 from cotton rat IL-13, fused to 5-His-tag at C-terminal expressed in CHO cell line.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 3-18mg/ml.
Endotoxin: ~1EU/1ug (LAL)
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.