Home  >  Products  >  IKK epsilon (IKKe, IkappaB Kinase epsilon, IKBKE Protein, IKK iota, IKKi, IKK Related Kinase epsilon, Inducible IkappaB Kinase, Inhibitor of kappa Light Polypeptide Gene Enhancer in B cells Kinase epsilon, Inhibitor of Nuclear Factor kappa B Kinase Subuni
IKK epsilon (IKKe, IkappaB Kinase epsilon, IKBKE Protein, IKK iota, IKKi, IKK Related Kinase epsilon, Inducible IkappaB Kinase, Inhibitor of kappa Light Polypeptide Gene Enhancer in B cells Kinase epsilon, Inhibitor of Nuclear Factor kappa B Kinase Subuni

IKK epsilon (IKKe, IkappaB Kinase epsilon, IKBKE Protein, IKK iota, IKKi, IKK Related Kinase epsilon, Inducible IkappaB Kinase, Inhibitor of kappa Light Polypeptide Gene Enhancer in B cells Kinase epsilon, Inhibitor of Nuclear Factor kappa B Kinase Subuni

Cat no: I3000-62C

Supplier: United States Biological
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NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using a subtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wild type IKKi in cells phosphorylates Ser32 and Ser36 of IkBa. Positive Control: 293, Jurkat, or NIH 3T3 Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I3000-62C
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by immunoaffinity chromatography.
Alternative names: EC=2.7.11.10, IKK-I
Additional info: In WB, a 80kD band is observed. Species Crossreactivity: Human, Mouse, Rat

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