Home  >  Products  >  IKK alpha (Biotin) (Azide Free) (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix Ubiquitous Kinase, CHUK1, IkappaB Kinase 1, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor kappa B Kinas

IKK alpha (Biotin) (Azide Free) (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix Ubiquitous Kinase, CHUK1, IkappaB Kinase 1, IKBKa, IKK1, Inhibitor Of kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor kappa B Kinas

Cat no: I3000-07C1


Supplier: United States Biological
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NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1)(1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns (2,3). Applications: Suitable for use in Western Blot and immunoprepcipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-2ug/ml. Western Blot: 1ug/ml. Detects a band at ~85kD. Optimal dilution determined by the researcher. Positive Controls: Daudi, NIH 3T3, or Raw cells Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I3000-07C1
Reactivities: Human, Mouse, Non-Human Primate
Hosts: Mouse
Applications: ELISA, Western Blot
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in PBS. No preservative added. Labeled with Biotin. Also available with azide. See I3000-07C2.
P type: Mab
Isotype: IgG1
Purity: Purified
References: 1.
Additional info: Recognizes human IKK alpha. Species Crossreactivity: mouse and monkey.

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