Home  >  Products  >  IkB alpha, phosphorylated (Ser32/Ser36) (I-kappa-B alpha, I(kappa)B(alpha), IkappaBalpha, IkB-alpha, IkBa, Major Histocompatibility Complex Enhancer Binding Protein MAD3, MAD-3, NF-kappa-B Inhibitor alpha, NFKBIa, NFKBI, Nuclear Factor of kappa Light Chai
IkB alpha, phosphorylated (Ser32/Ser36) (I-kappa-B alpha, I(kappa)B(alpha), IkappaBalpha, IkB-alpha, IkBa, Major Histocompatibility Complex Enhancer Binding Protein MAD3, MAD-3, NF-kappa-B Inhibitor alpha, NFKBIa, NFKBI, Nuclear Factor of kappa Light Chai

IkB alpha, phosphorylated (Ser32/Ser36) (I-kappa-B alpha, I(kappa)B(alpha), IkappaBalpha, IkB-alpha, IkBa, Major Histocompatibility Complex Enhancer Binding Protein MAD3, MAD-3, NF-kappa-B Inhibitor alpha, NFKBIa, NFKBI, Nuclear Factor of kappa Light Chai

Cat no: I2500-03F1

Supplier: United States Biological
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NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: TNFa treated Jurkat Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: I2500-03F1
Reactivities: Human, Mouse
Hosts: Mouse
Applications: ELISA
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in PBS containing 0.05% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG1,k
Purity: Serum
Additional info: Recognizes IkB alpha when phosphorylated at Ser32 and Ser36. Species Crossreactivity: Human, Mouse.

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