Home  >  Products  >  Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Member of PAS Protein 1, Mem

Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Member of PAS Protein 1, Mem

Cat no: H9806-01


Supplier: United States Biological
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The HIF1A gene encodes the HIF-1alpha subunit of hypoxia-inducible factor 1, a transcription factor that is essential for cardiovascular development and systemic O2 homeostasis (1). HIF-1 is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in hypoxic cells. It has been shown that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined by its presence in the in the mammalian ARNT and AHR proteins. HIF-1 alpha is most closely related to Sim (2). HIF-1 binds to cis-acting hypoxia-response elements within the erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in hypoxic cells. Results indicate that increased transcriptional activity mediated by HIF-1 in hypoxic cells results from both increased HIF-1alpha protein levels and increased activity of HIF-1alpha transactivation domains (3). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: H9806-01
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant.
References: 1. Iyer NV, et al. Genomics 52(2):159-65, 1998. 2. Wang GL, et al. Proc Natl Acad Sci 92(12):5510-4, 1995. 3. Jiang BH, et al. J Biol Chem 272(31):19253-60, 1997.
Additional info: Recognizes human Hypoxia Inducible Factor 1a. Species Crossreactivity: Rat.

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