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Hsp90 (HSP90AA1, HSP90A, HSPCAL1, HSPCAL4, HSPN, HSP86, HSPCA, Hsp89, HSP90N, LAP2, HSPC1, FLJ31884)

Cat no: H1831-55K

Supplier: United States Biological
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Hsp90 (also known as Hsp82, Hsp83, or Hsp89), a 90kD protein, is the most abundant protein in the cytosolic fraction in many cell types. The high level of Hsp90 in many cell types suggests that Hsp90 may play a general role in the cell, but little is known about its general function. Hsp90 has no known enzymatic activity, and thus it has been presumed that Hsp90 may function through protein-protein interactions. Hsp90 exists in a dimeric form and has been observed to bind to several cellular proteins such as retro-virus kinases (PP60v-src), steroid receptors, heme-regulated protein kinase, actin and tubulin. In this regard, Hsp90, like Hsp70, may function as a "molecular chaperone". Hsp90 exists as two isoforms referred to as Hsp86 and HSP84 in murine cells or Hsp90a and Hsp90b in human cells. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunoprecipitation: 4-8ug/ml Immunohistochemistry: 10-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: H1831-55K
Reactivities: Human, Mouse, Rat, Bovine, Non-Human Primate, Rabbit
Hosts: Mouse
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 1mg/ml BSA, 0.2% thimerosal, 50% glycerol.
P type: Mab
Isotype: IgG1
Purity: Purified by affinity chromatography.
Additional info: Recognizes mouse Hsp90 at ~90kD. Species Crossreactivity: human, rat, monkey, hamster, rabbit and bovine.

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