HSF 1 responds to cellular stress signals such as heat, heavy metals and oxidative reagents. It binds to heat shock response elements in the upstream region of heat shock gene and activates transcription. Upon activation, HSF 1 is trimerized and is localized in the nucleus where it binds the DNA.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.