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Histone H3, acetylated, Immunoprecipitation Assay Kit, BioAssay(TM)

Cat no: H5110-13Y

Supplier: United States Biological
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The assay kit contains reagents optimized for immunoprecipitation of transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR or Southern slot-blot analysis, using promotor specific primers or probe, is recommended. Applications: immunoprecipitation of chromatin using anti-Acetyl-Histone H3 Capacity: 22 assays Components: Anti-Acetylated Histone H3 ssDNA/Protein A Agarose all buffers and solutions Other components required but not included as part of kit are: Reagents Equipment Cells, stimulated or treated as appropriate for the experimental system 37% Formaldehyde PBS Dry ice Elution buffer: 1% SDS, 0.1M NaHCO3 Molecular Biology grade Proteinase K Glycogen or tRNA 50% Phenol/50%Chloroform (containing 1% isoamyl alcohol) 95% Ethanol 70% Ethanol vortex mixer rotating wheel/platform shaking incubator timer variable volume (5-1000l) pipettes + tips microfuge variable temperature water bath humidified 37C incubator cell scraper sonicator Storage and Stability: Stable for 1 year at 4 degrees C from date of shipment.
Catalogue number: H5110-13Y
Applications: Immunoprecipitation
Size: 1Kit
References: Alberts, A. S., et al., Cell. 92: 475, 1998 Braunstein, M., et al., Genes & Devel. 7: 592, 1993 Braunstein, M., et al., Mol. Cell. Biol. 16: 4349, 1996. Luo, R.X.., et al., Cell. 92: 463, 1998

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