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Histone H2AX (Histone H2A.x, H2a/x, H2AX, H2AFX)

Cat no: H5110-03Y

Supplier: United States Biological
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Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2500 Immunofluorescence: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: H5110-03Y
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, 30% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes human H2AX. Species Crossreactivity: mouse.

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