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Hepcidin, pro, Human, Control Peptide (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Cat no: H2009-01A

Supplier: United States Biological
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An 11aa peptide sequence from the N-terminus of mouse Pro-HEPC. Sequences is 91% conserved in rat and 63% in human pro-HEPC. This sequence is removed from the mature 20-25 secreted forms of HEPC, so antibodies will not react with the secreted forms of HEPC. Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The signal peptide is cleaved to produce pro-HEPC, which is further processed to generate the mature secreted form of hepcidin (C-terminal 20, 22 or 25-aa HEPC). In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is up-regulated by lipopolysaccharides (LPS). Control peptide, because of its small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10ug control peptide per 1ug of IgG or 1ul of antiserum) experiments to demonstrate antibody specificity. Full length, human 25-aa, 20-aa HEPC and mouse 25-aa HEPC are also available for various studies. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: H2009-01A
Reactivities: Mouse
Applications: ELISA, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2
Purity: Highly purified
References: 1. Pigeon, C., et al., JBC 276: 7811-7819 (2001). 2. Park, C.H., et al., JBC 276: 7806-7810 (2001). 3. Krause, A., et al., FEBS Lett. 480: 147-150 (2000). 4. Fleming, R.E. and Sly, W.S., PNAS 99: 8160-8162 (2001). 5. Nicolas, G., et al., PNAS 99: 4596-4601 (2002).

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