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HCCS (CCHL, Cytochrome c-type Heme Lyase, Holocytochrome c-type Synthase)

Cat no: H1826-80


Supplier: United States Biological
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The precursor of cytochrome c, apocytochrome c, is synthesized in the cytoplasm. Upon translocation into mitochondria, cytochrome c acquires a heme moiety required for functionality in the mitochondrial respiration chain. The heme-bound form of cytochrome c is called holocytochrome c. When released into the cytosol, cytochrome c can activate caspases responsible for apoptosis. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: H1826-80
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG1
Purity: Purified by affinity chromatography.
Additional info: Recognizes human Holocytochrome c at ~12kD. Species Crossreactivity: mouse and rat.

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