This ELISA kit is intended for the quantitative determination of human Haptoglobin levels in serum or other biological samples.
Range of Detection:
0.27-200ng/ml
Kit Components:
H1820-04A Coating Antibody, 1x1vial, Affinity-purified Chicken anti-Human Haptoglobin
H18209-04B Calibrator, 1x1vial, Pure Human Haptoglobin Antigen
H1820-04C Detection Antibody (HRP), 1x1vial, Affinity-purified Chicken anti-Human Haptoglobin - HRP Conjugate
Assay Condition:
The kit performance has been optimized for the stated protocol using the materials listed and standard dilutions from 0.27-200ng/ml of human Haptoglobin. For alternative assay conditions, the operator must determine appropriate dilutions of reagents. ELISA assay reactivity is sensitive to any variation in operator, pipetting and washing techniques, incubation time or temperature, composition of reagents, and kit age. Adjustments may be required to position the standard curve and/or samples in the desired detection range.
Specificity:
By immunoelectrophoresis and ELISA the antibodies in this kit react specifically with human Haptoglobin, not with other human serum proteins. The following factors prepared in the detection range of this kit, 0.27-200ng/ml were assayed and exhibited no cross-reactivity or interference: Human Serum Albumin, Human Transferrin, Human IgG.
Storage and Stability:
Store at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Country of Origin:
USA
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