CXCL3/GROg is a small cytokine belonging to the CXC chemokine family. CXCL3 controls migration and adhesion of monocytes and mediates it effects on its target cell by interacting with a cell surface chemokine receptor called CXCR2. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.
GRO gamma Recombinant, Human is a 7.9kD protein containing 73 amino acid residues.
Source: E. coli
Form: Lyophilized protein without additives.
Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0 degrees C. Reconstituted rat GRO-g should be stored in working aliquots at -20 degrees C.
Purity: (same/more than) 98% by SDS-PAGE and HPLC analyses.
Endotoxin: (same/less than) 0.1ng/ug.
Reconstitution: Soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in water to a concentration of 100ng/ul. This solution can be diluted into water or other buffered solutions or stored at -20 degrees C for future use.
Biological Activity: Maximal chemotactic activity on rat neutrophils is assayed in a modified Boyden chamber at 50ng/ml.
Responding Cells (partial list): Neutrophils
Concentration Range for most in vitro applications: 10-100ng/ml