Rat GRO-b (Rat MIP2) promotes neutrophil chemotaxis and degranulation. Rat GROb is a 7.9kD protein containing 73 amino acid residues.
Source: E. coli
Form: Lyophilized protein with no additives.
Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0 degrees C. Reconstituted rat GRO-b should be stored in working aliquots at -20 degrees C.
Purity: Greater than 98% by SDS-PAGE and HPLC analyses.
Endotoxin: (same/less than) 0.1ng/ug
Reconstitution: Lyophilized rat GRO-b is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in water to a concentration of 100 ng/ul. This solution can be diluted into water or other buffered solutions or stored at -20 degrees C for future use.
Biological Activity: The maximal chemotactic activity of rat GRO-b on rat neutrophils as assayed in a modified Boyden chamber was achieved at 50ng/ml.
Responding Cells (partial list): Neutrophils
Concentration Range for most in vitro applications: 10-100ng/ml