Three human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.
Recombinant Human GRO-alpha produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7811 Dalton. Recombinant Human GRO-alpha is purified by proprietary chromatographic techniques.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Ala-Thr.
Recombinant Melanoma growth stimulatory activity is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human neutrophils cells using a conc. of 10.0-100.0 ng/ml.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of MGSA as a Reference Standard.
It is recommended to reconstitute the lyophilized GRO-alpha in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized MGSA/GRO-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MGSA/GRO-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.