C-GPAGPRELAEHE, from the C-Terminus of human GRIK5.
Control peptide corresponding to polyclonal antibody, Catalog#G4005-96G, goat x human.
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik5, also known as kainate-preferring glutamate recptor subunit KA2, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik5 is highly homologous to the related ionotrophic glutamate receptor Grik4 (also known as KA1). Like Grik4, Grik5 does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface. This Grik5 antibody does not cross-react with Grik4.
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Human Brain (Frontal Cortex and Hippocampus) lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking (see corresponding antibody G4005-96G):
Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.