Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis.
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Immunohistochemistry (Paraffin): 1:50-1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.
Granzyme B, is expressed within the granules of cytolytic lymphocytes (CTLs).
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary Reagents:
I1904-65W IgG, F(ab