GM-CSF is a 23kD glycoprotein with diverse effects on immune and non-immune cells. It induces differentiation of granulocyte, macrophage and eosinophil precursor cells. Proliferation of monocyte-macrophages, T lymphocytes, keratinocytes and endothelial cells is stimulated by GM-CSF. In addition, GM-CSF alters the functional properties of mature granulocytes, macrophages, eosinophils and basophils. GM-CSF is produced by T lymphocytes, macrophages and several cell types in extreme dullary sites, where it may act in a paracrine manner to regulate the local response to antigenic challenge.
Applications:
Suitable for inhibition of biological activity of GM-CSF in functional studies and for immuno assay, Western Blot, Immunoprecipitation, and Immunohistochemistry (frozen). Other applications not tested.
Recommended Dilution:
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at-20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 0.5 ml sterile distilled or de-ionized water . Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.