Home  >  Products  >  Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sa
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sa

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sa

Cat no: G8951-15NX

Supplier: United States Biological
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Applications: ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human GM-CSF protein levels.1-4 Purified antibody should be paired with biotinylated antibody as the detection antibody, with purified recombinant human GM-CSF as the standard. Immunoprecipitation / Western Blotting: The purified antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of (same/less than) 100ng/lane of recombinant human GM-CSF under reducing conditions. Neutralization: The LE/AF form of the antibody can be used for neutralizing human GM-CSF bioactivity. A suitable LE/AF Rat IgG2a should be used as an isotype control. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8951-15NX
Reactivities: Human
Hosts: Rat
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 500ug
Form: Supplied as a liquid in PBS. No preservative added.
P type: Mab
Isotype: IgG2a,k
Purity: Purified
References: 1. Bacchetta, R., T. de Wall Malefyt, H. Yssel, J.S. Abrams, J.E. de Vries, H. Spits, and M.G. Roncarolo. 1990. J. Immunol. 144:902. 2. Kita, H., T. Ohnishi, Y. Okubo, D. Weller, J.S. Abrams, and G.J. Gleich. 1991. J. Exp. Med. 174:745. 3. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5. 4. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133.
Additional info: Recognizes human granulocyte/macrophage colony stimulating factor (GM-CSF).

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