G418 Sulfate is an aminoglycoside related to Gentamycin, used as a selective agent in bacterial genetics. It is toxic to yeast, bacteria, many plant and mammalian cells, helminths and protozoans.
Appearance:
White, crystalline powder
Solubility:
Colorless, clear, complete
Potency:
(same/more than) 620(u)ug/mg
Suggested Working Concentration: 100-800ug/ml
Mammalian Selection:
400ug/ml
Maintenance:
200ug/ml
Absorbance (1mg/ml):
280nm: (same/less than) 0.015
570nm: (same/less than) 0.10
Water (KF):
(same/less than)12%
Specific Rotation:
+104 degrees to +121C
pH:
5.0-6.0
Reconstitution:
Reconstitute with water or ethanol to a desired stock concentration. Sterilize by filtration through a 0.2um filter, aliquot and freeze at -20 degrees C.
Storage and Stability:
Powder: Store at 4C. Avoid moisture uptake, as this can decrease potency. Stable for 12 months.
Stock solution: Aliquot and store at -20 degrees C. Stable for 3-6months -20 degrees C.