In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leukocytes. F4213-01 has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation
giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat, 1:10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.