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Fas (CD95, APO-1) (FITC)

Cat no: F0019-59Z1


Supplier: United States Biological
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CD95, also known as Fas and Apo-1, is a 40-50kD type I transmembrane glycoprotein and a member of the tumor necrosis factor receptor superfamily. It is expressed by activated lymphocytes, monocytes, neutrophils, fibroblasts and cell lines. Fas ligand binding to CD95 induces apoptosis in activated mature lymphocytes thereby playing a role in maintaining peripheral tolerance. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and In vitro induction of apoptosis. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Catalogue number: F0019-59Z1
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 100Tests
Form: Supplied as a liquid in PBS, sodium azide. Labeled with FITC.
P type: Mab
Isotype: IgG2a
Purity: Purified
References: 1. Schlossman, S., et al., eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.2. Kishimoto, T., A.E.G. von dern Borne, S.M. Goyert, D.Y. Mason, M. Miyasaka, L. Moretta, K. Okumura, S. \ Shaw, T.A. Springer, K. Sugamura, and H. Zola, eds. 1998. Leukocyte Typing VI: White Cell Differentiation Antigens, Academic Press, New York. 3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD95 Section, Academic Press, New York, p. 363. 4. Nagata, S., and P. Golstein. 1995. Science 267:3378. 5. van Parijs, L., and A.K. Abbas. 1996. Curr. Opin. Immunol. 8:355.
Additional info: Recognizes human Fas (CD95, APO-1).

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