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Erk1,2, Phosphorylated Pathway BioAssay(TM) Sample Kit

Erk1,2, Phosphorylated Pathway BioAssay(TM) Sample Kit

Cat no: E3452-50

Supplier: United States Biological
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, cell differentiation, cell movement and cell death. MAPK signaling cascades are organized hierarchically into three-tiered modules. MAPKs are phosphorylated and activated by MAPKkinases (MAPKKs), which in turn are phosphorylated and activated by MAPKK-kinases (MAPKKKs). The MAPKKKs are in turn activated by interaction with a family of small GTPases and/or other protein kinases connecting the MAPK module to the cell surface receptor or external stimuli. The Phospho-Erk1/2 Pathway Sampler Kit provides a fast and economical means to evaluate the activation status of the entire Erk1/2 pathway. The kit includes enough primary and secondary antibodies to perform four Western mini-blot experiments, as well as a specific inhibitor of MEK1/2 (U0126).
Catalogue number: E3452-50
Size: 5x40ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Purity: Purified by protein A and peptide affinity chromatography.
References: 1. Lewis, T.S. et al. (1998) Adv. Cancer Res. 74:49
Additional info: Each antibody in the Phospho-Erk1/2 Pathway Sampler Kit recognizes only the phosphorylated form of its specific target.

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