Extracellular signal regulated kinase (ERK), also known as mitogen activated protein kinase (MAPK), is a family with two homologous members; ERK1 (44kD) and ERK2 (42kD). This antibody reacts with the active forms of these proteins that have specific phosphorylated Threonine and Tyrosine residues within a Thr-Glu-Tyr motif (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2)
Suitable for use in Western Blot . Other applications not tested.
Western Blot:. 0.25-0.50ug/ml. Band expected at ~43kD for human Erk1 and ~41kD for human Erk2.
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.