ELISA Development Kit, BioAssay,(TM) Mouse is designed to detect and quantitate antigen-specific antibodies in serums and other samples using a proprietary one-hour indirect ELISA procedure. Ideal for large-scale antibody titer determination. This kit allows user to perform a complete ELISA from antigen coating to signal development in just one hour. The one-hour protocol is contrasted with a classical five-hour ELISA procedure.
Easy to Use:
1. Coat the plate with an antigen (protein or peptide) in the coating buffer for five minutes.
2. Block the plate with pretreatment solution for five minutes
3. After the 30-minute binding phase, treat the plate with the ELISA solution for 20 minutes.
4. The plate can then be developed with the custom TMB solution included.
Kit Components:
The kit contains all necessary reagents and buffers for performing indirect ELISA using ten 96-well plates.
Dilution Solution, 1x125ml
ELISA Solution, 1x125ml
TMB, 1x110ml
Pretreat A Solution, 1x125ml
Pretreat B Solution, 1x125ml
Stop Solution, 1x125ml
20X Wash Solution, 1x125ml
2X Coating Buffer, 1x 125ml
Key Features:
Fast: This kit cuts indirect ELISA time down to one hour.
Sensitive: Same as or better than standard procedures.
Reproducible: The kit produces highly reproducible results.
Minimal optimization: Needs significantly less optimization than classical ELISA methods.
Storage and Stability:
Store the kit at 4 degrees C. It will remain stable for six months. Do not freeze the kit or any of its components.
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