ELISA Development Kit, BioAssay,(TM) Goat is designed to detect and quantitate antigen-specific antibodies in serums and other samples using a proprietary one-hour indirect ELISA procedure. Ideal for large-scale antibody titer determination. This kit allows user to perform a complete ELISA from antigen coating to signal development in just one hour. The one-hour protocol is contrasted with a classical five-hour ELISA procedure.
Easy to Use:
1. Coat the plate with an antigen (protein or peptide) in the coating buffer for five minutes.
2. Block the plate with pretreatment solution for five minutes
3. After the 30-minute binding phase, treat the plate with the ELISA solution for 20 minutes.
4. The plate can then be developed with the custom TMB solution included.
The kit contains all necessary reagents and buffers for performing indirect ELISA using ten 96-well plates.
Dilution Solution, 1x125ml
ELISA Solution, 1x125ml
Pretreat A Solution, 1x125ml
Pretreat B Solution, 1x 125ml
Stop Solution, 1x125ml
20X Wash Solution, 1x125ml
2X Coating Buffer, 1x 125ml
Fast: This kit cuts indirect ELISA time down to one hour.
Sensitive: Same as or better than standard procedures.
Reproducible: The kit produces highly reproducible results.
Minimal optimization: Needs significantly less optimization than classical ELISA methods.
Storage and Stability:
Store the kit at 4 degrees C. It will remain stable for six months. Do not freeze the kit or any of its components.