EDG1 (Endothelial Cell Differentiation Gene 1, EDG 1, EDG-1, Endothelial Differentiation Sphingolipid G Protein Coupled Receptor 1, CHEDG1, D1S3362, G Protein Coupled Sphingolipid Receptor, Sphingosine 1-phosphate Receptor Edg-1, Sphingosine 1-phosphate R

Cat no: E2208-01G

Supplier: United States Biological

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Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling. At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform. Applications: Suitable for use in Elisa and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse Thymus Tissue Lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	E2208-01G
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	ELISA, Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, 0.02% sodium azide.
P type:	Pab
Purity:	Purified by immunoaffinity chromatography.
References:	1. Miyasaka M and Tanaka T. Lymphocyte tracking across high endothelial venules: dogmas and enigmas. Nat. Rev. Immunol. 2004; 3:360-70. 2. Mandala S, Hajdu R, Bergstrom J, et al. Alteration of lymphocyte tracking by sphingosine-1-phosphate receptor agonists. Science 2002; 296:346-9. 3. Ledgerwood LG, Lal G, Zhang N, et al. The sphingosine-1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics. Nat. Immunol. 2008; 9:42-53. 4. Pham TH, Okada T, Matloubian M, et al. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity 2008; 28:122-33.
Additional info:	Recognizes human S1P1. Species Crossreactivity: Mouse and rat.

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EDG1 (Endothelial Cell Differentiation Gene 1, EDG 1, EDG-1, Endothelial Differentiation Sphingolipid G Protein Coupled Receptor 1, CHEDG1, D1S3362, G Protein Coupled Sphingolipid Receptor, Sphingosine 1-phosphate Receptor Edg-1, Sphingosine 1-phosphate R

Cat no: E2208-01G

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