Estrogen receptor binding site associated antigen 9, also known as EBAG9, is a type III transmembrane protein that is predominantly located in the Golgi. This protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers, such as advanced breast and prostate cancers. As the EBAG9 acts to inhibit the growth of receptor-binding cells and induced apoptosis of immune cells, cancer cells might evade immune surveillance. So immunodetection of EBAG9 expression can be a negative prognostic indicator.
Recombinant human EBAG9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Recombinant corresponding to aa28-213 of human EBAG9 (His-tagged) expressed in E. coli.
MGSSHHHHHH SSGLVPRGSH MRSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 3ug/ml 15% SDS-PAGE, ~23.4kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Can be stored at +4 degrees C short term (1-2 weeks). For long term storage, aliquot and store at -20 degrees C or -70 degrees C. Avoid repeated freezing and thawing cycles.