Contains 11 discrete fragments ranging from 50bp to 1000bp with a double intensity reference band at 500bp. Suitable for size determination of digested DNA.
The marker yields the following 11 discrete fragments: 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50.
Concentration:
0.05mg/ml
Quantity of DNA in band:
Fragment (bp) ng/10ul
1000 76
900 68
800 61
700 53
600 46
500 75
400 30
300 22
200 30
100 22
50 15
Recommendations for Use:
1. Vortex gently just prior to use.
2. Do not heat before loading.
3. Apply 10ul of DNA Ladder per well.
4. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5ug/ml ethidium bromide for 30-40 minutes.
*Note: This ladder was standardized using 10ul of DNA per lane on a 0.8cm thick, 13x15cm, 1% agarose gel rrun in TAE buffer.
Storage and Stability:
May be stored at -20 degrees C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.