Cytokine Array Panel, Mouse, BioAssay(TM)

For the parallel determination of the relative levels of selected mouse cytokines and chemokines. Cytokines and chemokines are extracellular signaling molecules that mediate cell-cell communication. They are released from cells and have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity and inflammation. In most biological processes, multiple cytokines operate in a large network, where the action of one cytokine is regulated by the presence or absence of other cytokines. Measuring cytokines present in biological samples one at a time is tedious and requires large sample volumes. Mouse Cytokine Array Panel A is an excellent tool for simultaneously profiling the relative levels of multiple cytokines in a single sample. Principle: Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the Mouse Cytokine Array Panel A. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Following a wash to remove unbound material, Streptavidin-HRP and chemiluminescent detection reagents are added sequentially. Light is produced at each spot in proportion to the amount of cytokine bound. Refer to the Appendix on page 12 for a list and coordinates of target capture antibodies. Kit Components: Store the unopened kit at 2-8 degrees C. Do not use past expiration date. Component Part # StorageConditions Amount Provided Mouse Cytokine Array Panel A 2-8 degrees C 4 membranes Array Buffer 4 2-8 degrees C 1 vial (21ml) Array Buffer 5 2-8 degrees C 1 vial (21mLl) Array Buffer 6 2-8 degrees C 1 vial (21ml) Wash Buffer Concentrate, 25X 2-8 degrees C 2 vials (21ml each) Antibody Detection Cocktail, Mouse Cytokine Array Panel A 2-8 degrees C 1 vial Streptavidin-HRP 2-8 degrees C 1 vial 4-Well Rectangular Multi-dish 1 dish Transparency Overlay Template 1 template