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Cytokeratin 18, Caspase-cleaved (FITC)

Cat no: C9097-23W2

Supplier: United States Biological
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Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder, trophoblasts. Cytokeratin 18, Caspase-cleaved monoclonal antibody has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site (M30-neoepitope) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells. Applications: Suitable for use in Immunocytochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:100 Immunocytochemistry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C9097-23W2
Reactivities: Human, Bovine, Non-Human Primate
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry
Size: 200Tests
Form: Supplied as a liquid in PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified supernatant
References: 1. M.P. Leers, et al.; J. Pathol. 187, 567 (1999) 2. C. J. Larson, et al.; Cytometry A. 62, 46 (2004) 3. N. Marceau; Methods Cell Biol. 78, 95 (2004) 4. C. Vogt Isaksen; Pediatr. Dev. Pathol. 7, 433 (2004) 5. K. Asai, et al.; Scand. J. Gastroenterol. 40, 326 (2005) 6. K. Dvorakova, et al.; Am. J. Gastroenterol. 100, 424 (2005)
Additional info: Recognizes the human CK18-Asp396 neo-epitope of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.

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