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Cytokeratin 18, Caspase-cleaved

Cat no: C9097-23W

Supplier: United States Biological
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Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder and trophoblasts. Cytokeratin 18, Caspase-cleaved monoclonal antibody has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site (M30-neoepitope) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry Immunohistochemistry and Western Blot. Other applicatiions not tested. Recommended Dilutions: Flow Cytometry: 1:100 Immunocytochemistry: 1:100 Immunohistochemistry (Frozen, Paraffin): 1:100. Western Blot: 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C9097-23W
Reactivities: Human, Bovine, Non-Human Primate
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 200Tests
Form: Supplied as a liquid in PBS, 0.1% BSA, PEG, sucrose, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified
References: 1. M.P. Leers, et al.; J. Pathol. 187, 567 (1999) 2. C. J. Larson, et al.; Cytometry A. 62, 46 (2004) 3. N. Marceau; Methods Cell Biol. 78, 95 (2004) 4. C. Vogt Isaksen; Pediatr. Dev. Pathol. 7, 433 (2004) 5. K. Asai, et al.; Scand. J. Gastroenterol. 40, 326 (2005)
Additional info: Recognizes the human CK18-Asp396 neo-epitope (M30) of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.

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