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Cytokeratin 18, Caspase-cleaved (Biotin)

Cat no: C9097-23W1


Supplier: United States Biological
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Cytokeratin-18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder and trophoblasts. Cytokeratin 18, Caspase-cleaved monoclonal antibody has been shown in numerous independent studies to be specific to the CK18 Asp396 caspase cleavage site (M30-neoepitope) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100 Immunocytochemistry: 1:100 Immunohistochemistry (Paraffin and Frozen): 1:100 Western Blot: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C9097-23W1
Reactivities: Human, Bovine, Non-Human Primate
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Conjugates: Biotin
Size: 200Tests
Form: Supplied as a liquid in PBS, 0.1% BSA, PEG, sucrose and 0.09% sodium azide. Labeled with biotin.
P type: Mab
Isotype: IgG2b
Purity: Purified supernatant
References: 1. M.P. Leers, et al. J. Pathol. 187, 567 (1999). 2. C. J. Larson, et al. Cytometry A. 62, 46 (2004). 3. N. Marceau. Methods Cell Biol. 78, 95 (2004). 4. Pediatr. Dev. Pathol. 7, 433 (2004). 5. K. Asai, et al. Scand. J. Gastroenterol. 40, 326 (2005). 6. K. Dvorakova, et al. Am. J. Gastroenterol. 100, 424 (2005). 7. P. Fickert, et al. J. Hepatol. 42, 378 (2005). 8. P.Y. Fong, et al.; Cancer 103, 268 (2005). 9. H. Holubec, et al.; J. Histochem. Cytochem. 53, 229 (2005). 10. I. Kausch, et al.; Eur. Urol. 47, 703 (2005). 11. J.J. Koornstra, et al. Eur. J. Cancer 41, 1195 (2005). 12. H. Lee, et al. Placenta 26, 226 (2005). 13. C. Miquel, et al. Am. J. Clin. Pathol. 123, 562 (2005). 14. Q. Qiu, et al.; J. Reprod. Immunol. 65, 121 (2005). 15. N. Vigneswaran, et al.; Int. J. Oncol. 26, 103 (2005). 16. B. Vijayalakshmi, et al. Free Radic. Biol. Med. 38, 1614 (2005). 17. L. Vormittag, et al.; Oncol. Rep. 13, 207 (2005).
Additional info: Recognizes the human CK18-Asp396 neo-epitope of caspase-cleaved CK18. Species Crossreactivity: monkey and bovine. Does not crossreact with intact CK18.

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