CRISP-3 belongs to the large CRISP family of secreted proteins with a characteristic C-terminal cysteine-rich (CR) domain. Members of the CRISP family contain three conserved domains: a plant pathogenesis related group 1 (PR-1) domain, a short hinge region, and a CR domain. CRISP-3 is expressed in salivary glands, prostate, pancreas, and in the secretory granules of neutrophilic granulocytes. In plasma, CRISP-3 circulates in a complex with alpha B-glycoprotein, a member of the Ig superfamily. CRISPs are characterized by a cysteine-rich domain at the COOH terminal that form 8 intramolecular disulfide bonds. Mammalian members of the CRISP family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, post-testicular sperm maturation and capacitation to oocyte-sperm fusion. CRISP3 is epithelium-specific and found predominantly in salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. CRISP3 is up-regulated in malignant prostatic epithelium, therefore, it can be used as a potential prostate cancer biomarker.
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
ELISA (competitive): 1:200-1:400
Flow Cytometry: 1.2ug per 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.