Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S. Cystatin C, a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight 13260D, is composed of 146 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.
For the quantitative determination of human Cystatin C concentrations in cell culture supernates, serum, plasma, saliva, urine, and human milk.
Kit Components:
C8950-24A: Microtiter Plate, 1x96 wells
C8950-24B: Conjugate (HRP), 1x21ml
C8950-24C: Cystatin C Standard, 1x200ng
C8950-24D: Assay Diluent, 1x11ml
C8950-24E: Calibrator Diluent Concentrate, 1x21ml
C8950-24F: Wash Buffer Concentrate (25X), 1x21ml
C8950-24G: Color Reagent A (H2O2), 1x12.5ml
C8950-24H: Color Reagent B (TMB), 1x12.5ml
C8950-24J: Stop Solution, 1x6ml
Important note: Human Cystatin C Control not supplied but are available from United States Biological.
Storage and Stability:
Store all powders at 4 degrees C. Store reconstituted liquids at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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