Cystatin A (acid cysteine proteinase inhibitor, ACPI) is a natural inhibitor of cysteine proteinases and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. Human cystatin A consists of 98aa residues and belongs to cystatin subfamily I (stefins). Among the target enzymes inhibited by cystatin A are cathepsins B, C, H, L, and S, papain and actinidin. Cystatin A has also been detected in infiltrative breast carcinoma.
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Western Blot: 1-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.