The C-terminal-binding protein (CtBP) was originally identified as a cellular protein that interacts with a C-terminal motif of adenovirus E1A oncoproteins. CtBP is highly conserved in higher eukaryotes. Vertebrates contain two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates contain a single CtBP. CtBP family proteins share significant sequence homology with D-2-hydroxy acid dehydrogenases. Also, CtBP proteins associate with various proteins and functions as a co-repressor of transcription.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.
FO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.