COPS3 is a component of the COP9 signalosome complex (CSN), a complex involved in various
cellular and developmental processes.Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control (1, 2).Subunit 3 (COPS3) possesses kinase activity that phosphorylates regulators involved in signal transduction.It phosphorylates I kappa-B alpha, p105, and c-Jun.It acts as a docking site for complex-mediated phosphorylation (3).COPS3 is suggested as a specific negative regulator of TNF- induced NF-kappaB activation pathways because its over- expression inhibits NF-kappaB activation (4).
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Flow Cytometry: 1:20
Western Blot: 1:10,000-1:20,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.