Crk (p38) are adaptor proteins, composed mostly of SH2 and SH3 domains, which play a key role in cellular signaling by mediating the construction of protein complexes and also by managing cell proliferation, differentiation, and migration (1). They are critical integrators
of upstream signals for cell invasion and migration in human cancer cell lines. Crk is believed to be a regulator of invasive responses because increased levels of the protein have been
observed in multiple human cancers (2). In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth (3). Crk
associates with ERM family proteins (including ezrin, radixin, and moesin), activates RhoA, and promotes cell motility toward hyaluronic acid (4).
Applications:
Suitable for use in Immunocytochemistry, Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-2000
Immunocytochemistry: 1:50 -10
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.