Corticotropin-releasing hormone receptor 1 (CRHR-1) is a member of the Class B G protein-coupled receptor family that mediates the action of hypothalamic and peripheral CRH. It is the key regulator of the stress response in the hypothalamic-pituitary adrenal axis and an important functional mediator of the endocrine, cardiovascular, gastrointestinal, and immune systems. The other major endogenous ligand for CRHR-1 is urocortin 1. CRHR-1 is highly expressed in the cerebral cortex, striatum, amygdala, and cerebellum in rodents (1). In humans, transcripts are detected in cortex, brainstem, and pituitary, but not in placenta and peripheral blood lymphocytes (2). It is also expressed in human adrenal cortex and adrenal tumor cell line NCI-H295R (3), non-pregnant and pregnant myometrium (4), and weakly in placental tissues (5).
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 10ul labels 1x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.