The glucocorticoid Corticosterone is secreted by the adrenal gland in response to stress for mammalian species. Glucocorticoids also serve a function in the metabolism of fats, protein and carbohydrate and can be adminstered as an anti-inflammatory agent. Levels of both cortisol and corticosterone increase in mammalian species due to stressors. Only the corticosterone is the dominant glucocorticoid elevated in response to stress for both mice and rats as well as other non-human mammalian species. The actual stressors are not always indicative for the elevated levels of corticosterone but among them are climate changes, changes in environment, rapid temperature changes and changes in daily activity.
This ELISA was developed for the quantitation of corticosterone in relation to stressors on a test subject. This ELISA is specific for corticosterone with minimal cross reactivity from other key glucocorticoids. Measurement of cortcosterone is obtained with a competitive ELISA design. The corticosterone from the sample competes for a limited number of binding sites with the enzye conjugate. Readings can be taken at 650nm or 450nm with stop solution.
C7903-30A: Microplate, 1x96 wells
C7903-30C: Corticosterone Standard, 1x100ul
C7903-30D: Corticosterone (HRP), 1x150ul
C7903-30E: Substrate (TMB), 1x20ml
C7903-30F: ELISA Buffer, 1x30ml
C7903-30G: Wash Buffer (10X), 1x20ml
C7903-30H: Extraction Buffer (5X), 1x30ml
Storage and Stability:
Store at 4 degrees C. Stable for 6 months.