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Chick Embryo Extract, Ultrafiltrate (CEE)

Cat no: C3999

Supplier: United States Biological
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Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an ultrafiltration step with a 10kD MW cutoff to remove protein from the solution, producing a clear, amber liquid. This liquid is sterile-filtered. Animals are sourced from a specific USDA-Registered facility using a veterinary-inspected flock. Final processing at an ISO9000 facility. Chick Embryo Extract, Ultrafiltrate is protein free. It does not support the growth of viruses. Appearance: Pale-yellow, clear, solution Total Protein (Biuret): ~0.0g/dL Sterility (per 9CFR 113.26): Negative Mycoplasma (per 9CFR 113.26): Negative Osmolality: 260-360mOsm/Kg H2O Viral Testing Pullorum typhoid, Galisepticum, M. synoviae, M. meleagridis): Negative Recommended Dilutions: 1ml of extract/100ml of media Storage and Stability: Chick Embryo Extract, Ultrafiltrate is protein-free; thus, it will not break down. May be stored at RT. Stable for 12 months. Storage at 4 degrees C extends the stability for an additional 3-6 months. Some precipitate has been observed when frozen.
Catalogue number: C3999
Size: 20ml
References: US Biological application references: 1. Griffin, M.A. et al., (2004) J. Cell Science 117:5855-5863. 2. Clause, K.C. et al., (2010) Tissue Engineering Part C, 16:375-385. 3. Montemurro, T. et al., (2009) J Cell Mol Med. Mar 9, Epub. 4. Jiang, X. et al., (2009) Stem Cells Dev. 18:1059-1070. 5. Lokireddy, S. et al., (2011) Am J Physiol Cell Physiol, http://ajpcell.physiology.org/content/early/2011/09/01/ajpcell.00114.2011 OR http://www.ncbi.nlm.nih.gov/pubmed/21900687. 6. de la Garza-Rodea, A.S. et al., (2013) FASEB J. doi: 10.1096/fj.13-233155.

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