Intented Use:
This Human Chemerin ELISA kit is used for the non-radioactive quantification of Human Chemerin in serum, plasma, tissue extract and cell culture samples. One kit is sufficient to measure 38 unknown samples in duplicate.
Principle of the Procedure:
This assay is a Sandwich ELISA based, sequentially, on: 1) capture of Human Chemerin molecules from samples to the wells of a microtiter plate coated by a pre- titered amount of anti-Human Chemerin antibody, 2) wash away of unbound materials from samples, 3) binding of a biotinylated anti-human Chemerin antibody to the captured molecules, 4) wash away of unbound materials from samples, 5) conjugation of horseradish peroxidase to the immobilized biotinylated antibodies, 6) wash away of free enzyme conjugates, and 7) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate 3,3
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