Chk2 is the mammalian homolog of yeast Cds1/Rad53. In response to DNA damage, a checkpoint kinase ATM phosphorylates and activates Chk2, which in turn phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.