Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase (figure 1). Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161 (1-4). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (3,5). Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (3,4,6).
Suitable for use in Western Blotting. Other applications not tested.
Western blotting: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.