CDw92 (isoform 3), also known as CTL1, a 70kD multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine. Reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat-1:10. Use 10ul to label 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Spleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C. Stable for 12 months. Reconstitute with sterile ddH2O. Aliquot and store at 4 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.